abstract：:Heart and skeletal muscle cells rapidly lose potassium ions after withdrawal of oxygen. In myocardial ischemia, cellular release of potassium and interruption of extracellular washout produce a rapid and marked increase of extracellular K+ concentration. Harris et al. were the first to observe the coincidence of the K...

journal_title：Journal of molecular and cellular cardiology

authors： Kléber AG,Riegger CB,Janse MJ

更新日期：1987-10-01 00:00:00

abstract：:In the present investigation, the effects of forskolin on intracellular sodium activity were studied in quiescent and electrically stimulated cardiac Purkinje fibers from sheep using Na+-sensitive microelectrodes. Also assessed, were the effects of this promoter of cytosolic cAMP production on resting membrane potenti...

journal_title：Journal of molecular and cellular cardiology

authors： Zalups RK,Sheu SS

更新日期：1987-09-01 00:00:00

abstract：:Isolated and perfused rat hearts were examined by 2-dimensional gel electrophoresis and liquid scintillation counting to determine the effect of ischemia or perfusion temperature on protein synthesis. Isolated hearts were subjected to ischemia at either 4 degrees, 20 degrees, or 30 degrees C and then perfused at 37 de...

journal_title：Journal of molecular and cellular cardiology

authors： Currie RW

更新日期：1987-08-01 00:00:00

abstract：:The isometric twitch properties of papillary muscles from hearts of 30- to 53-day-old cardiomyopathic hamsters (BIO 14.6) were studied before and after exposure to the cardiac glycoside, ouabain. The diseased tissue was weakly responsive to ouabain (3 to 100 microM), as compared to a more appreciable positive inotropi...

journal_title：Journal of molecular and cellular cardiology

authors： Rossner KL,Mascarenhas DA

更新日期：1987-06-01 00:00:00

abstract：:We compared myocardial mechanics and myosin isozymes of right and left ventricular papillary muscles from adult (6 to 8 month old) male rats. Analysis of force velocity relations indicate that right ventricular papillary muscles contract more rapidly than left at light loads (2.68 +/- 0.13 vs 2.18 +/- 0.07 muscle leng...

journal_title：Journal of molecular and cellular cardiology

authors： Brooks WW,Bing OH,Blaustein AS,Allen PD

更新日期：1987-05-01 00:00:00

abstract：:This study describes the effect of oxygen radicals on the ultrastructure of the isolated Langendorff-perfused rat heart. Oxygen radicals were enzymatically generated by xanthine oxidase (0.025 U/ml) and hypoxanthine (0.96 mM). Hearts were perfusion-fixed for electron microscopy and stereological technique was performe...

journal_title：Journal of molecular and cellular cardiology

authors： Ytrehus K,Myklebust R,Olsen R,Mjøs OD

更新日期：1987-04-01 00:00:00

abstract：:The effect of isoproterenol on mechanical function was studied in the isolated arterially perfused heart of the fetal (21st and 28th day of gestation) and newborn rabbits. The inotropic effect of isoproterenol in the fetus was less than in the newborn. In contrast, myocardial cyclic AMP levels after isoproterenol infu...

journal_title：Journal of molecular and cellular cardiology

authors： Okuda H,Nakanishi T,Nakazawa M,Takao A

更新日期：1987-02-01 00:00:00

abstract：:Milrinone, a potent positive inotropic and vasodilating agent, has shown promise in the clinical treatment of congestive heart failure, but significant controversy about its mechanism of action exists. To approach these mechanistic problems in a non-innervated, non-diffusion-limited system, the effects of milrinone on...

journal_title：Journal of molecular and cellular cardiology

authors： Olson EM,Kim D,Smith TW,Marsh JD

更新日期：1987-01-01 00:00:00

abstract：:The effects of trimetazidine on membrane potentials and membrane currents of enzymatically isolated guinea-pig ventricular cells were studied with the use of giga-seal suction pipettes for patch clamp. Trimetazidine (3 X 10(-5) M) decreased the action potential duration from 433 +/- 179 ms (mean and S.D., n = 9) to 31...

journal_title：Journal of molecular and cellular cardiology

authors： Kiyosue T,Nakamura S,Arita M

更新日期：1986-12-01 00:00:00

abstract：:Immediate post-receptor events following alpha-adrenoceptor stimulation in cardiac ventricular muscle are still largely unknown. Since membrane redox systems appear to be present in cell plasma membranes and may be involved in trans-sarcolemma electron efflux, the possibility that Ca2+ inflow was controlled by electro...

journal_title：Journal of molecular and cellular cardiology

authors： Clark MG,Rattigan S

更新日期：1986-11-01 00:00:00

abstract：:The interaction of verapamil and other phenylalkylamine calcium channel blockers with the 1,4-dihydropyridine receptor was examined. Studies characterizing the interaction and relationship between calcium channel blocking potency and binding affinity were performed in rat myocardium. The 1,4-dihydropyridines, nifedipi...

journal_title：Journal of molecular and cellular cardiology

authors： Zobrist RH,Giacomini KM,Nelson WL,Giacomini JC

更新日期：1986-09-01 00:00:00

abstract：:The effects of the inhibition of phospholipid degradation and superoxide radical generation on prostaglandin synthesis associated with myocardial ischemia and reperfusion were studied in the isolated, in-situ pig heart model subjected to 60 mins of regional ischemia and a further 60 mins of hypothermic potassium cardi...

journal_title：Journal of molecular and cellular cardiology

authors： Otani H,Engelman RM,Rousou JA,Breyer RH,Das DK

更新日期：1986-09-01 00:00:00

abstract：:Basal levels of cyclic adenosine monophosphate (cAMP) were measured in embryonic chick hearts at various times during development. Basal cAMP was highest on incubation day 5 and decreased throughout the remaining incubation period. Cyclic AMP could not be stimulated above basal level by intravenous in ovo administrati...

journal_title：Journal of molecular and cellular cardiology

authors： Phillips MT,Kirby ML,Stewart DE

更新日期：1986-08-01 00:00:00

abstract：:Cardiac calcium channel activity is markedly increased by beta-adrenergic agents or calcium agonists such as Bay K 8644. The molecular mechanisms underlying these important modulatory effects have been studied with patch clamp techniques by several groups. This paper presents new experiments and reviews published evid...

journal_title：Journal of molecular and cellular cardiology

authors： Tsien RW,Bean BP,Hess P,Lansman JB,Nilius B,Nowycky MC

更新日期：1986-07-01 00:00:00

abstract：:The ability of different substrates to affect myocardial function is well established but the mechanism for this effect has yet to be determined. To explore this area further, the studies described below were designed to determine the effect of different metabolic substrates, glucose or pyruvate, on myocardial respons...

journal_title：Journal of molecular and cellular cardiology

authors： Snow TR

更新日期：1986-07-01 00:00:00

abstract：:A newly developed method was used to make a direct study of the influence of lanthanum on the subcellular distribution of isotopic and elemental calcium in the isolated perfused dog heart. The trivalent cation was introduced into the heart for the last 5 min of a 60 min perfusion period. Developed pressure and dP/dt w...

journal_title：Journal of molecular and cellular cardiology

authors： Kolbeck RC,Speir WA Jr

更新日期：1986-07-01 00:00:00

abstract：:Baroreflex control of heart rate was studied in conscious diabetic rats at 12, 24 and 48 weeks after the induction of diabetes with streptozotocin. Baseline blood pressure (mean arterial blood pressure) of diabetic rats was significantly lower at 12 weeks after the induction of diabetes when compared to age-matched co...

journal_title：Journal of molecular and cellular cardiology

authors： Chang KS,Lund DD

更新日期：1986-06-01 00:00:00

abstract：:The volume of blood in the left ventricular wall increases as coronary perfusion pressure or coronary blood flow increase, but it is unclear whether such an increase in volume can acutely alter the diastolic pressure-dimension relationship of the intact, working left ventricle. In 19 dogs, we measured left ventricular...

journal_title：Journal of molecular and cellular cardiology

authors： Verrier ED,Bristow JD,Hoffman JI

更新日期：1986-06-01 00:00:00

abstract：:Quin2 is a fluorescent Ca2+-sensitive indicator which in its acetoxymethyl ester form can be loaded into the cytosolic compartment of cells without any disruption of the plasma membrane. Quin2 has been used with many cell types to measure changes of [Ca2+]i in response to hormones and other stimuli. In this report we ...

journal_title：Journal of molecular and cellular cardiology

authors： Thomas AP,Selak M,Williamson JR

更新日期：1986-05-01 00:00:00

abstract：:Exposure to carbon monoxide (CO) during the fetal and neonatal period was used to evaluate cardiac ventricular regional weight and myocyte growth response to an increased hemodynamic load. Date-mated Sprague-Dawley rats inhaled 200 ppm CO from day 7 of pregnancy until parturition; another group of pregnant rats inhale...

journal_title：Journal of molecular and cellular cardiology

authors： Clubb FJ Jr,Penney DG,Baylerian MS,Bishop SP

更新日期：1986-05-01 00:00:00

abstract：:This study was designed to examine whether diltiazem, a calcium channel-blocker, inhibits the cardiac ultrastructural alterations induced by coronary occlusion with or without reperfusion, in dogs anesthetized with pentobarbital. The left anterior descending coronary artery (LAD) was completely occluded for 60 min wit...

journal_title：Journal of molecular and cellular cardiology

authors： Sashida H,Abiko Y

更新日期：1986-04-01 00:00:00

abstract：:Function and various parameters of myocardial substrate and energy metabolism were measured in preload-controlled isolated working guinea-pig hearts perfused with normoxic (95% O2) and hypoxic (30 to 45% O2) Krebs-Henseleit buffers ([Ca2+] = 1.25 mM). Energy-yielding substrates were glucose, pyruvate, lactate, and fat...

journal_title：Journal of molecular and cellular cardiology

authors： Bünger R,Swindall B,Brodie D,Zdunek D,Stiegler H,Walter G

更新日期：1986-04-01 00:00:00

abstract：:The histologic examination and planimetric evaluation of tissue slices has been so far the only available technique for studying the extent of experimental myocardial infarcts in long-term studies; however, this approach is rather time-consuming when the sample size is large. This study describes a new biochemical met...

journal_title：Journal of molecular and cellular cardiology

authors： Chiariello M,Ambrosio G,Cappelli-Bigazzi M,Perrone-Filardi P,Brigante F,Sifola C

更新日期：1986-03-01 00:00:00

abstract：:Previously, we demonstrated that acute exposure to superfusate containing verapamil suppresses action potential plateau to a greater degree in cells at the tip of the anterior papillary muscle compared to those at its base in normal cat left ventricle (LV) studied in tissue bath. To determine the effects of chronic pr...

journal_title：Journal of molecular and cellular cardiology

authors： Cameron JS,Miller LS,Kimura S,Kaiser CJ,Campbell DR,Kozlovskis PL,Gaide MS,Myerburg RJ,Bassett AL

更新日期：1986-02-01 00:00:00

abstract：:A reduced adaptation rate of the myocardium must, in many cases, be made responsible for heart insufficiency of old patients. The present investigations are aimed towards the question whether heart in old age is accompanied by a loss of myocardial cells and whether a polyploidisation of heart muscle cells takes place ...

journal_title：Journal of molecular and cellular cardiology

authors： Adler CP,Friedburg H

更新日期：1986-01-01 00:00:00

abstract：:A cardiac muscle sarcolemmal preparation, enriched in adenylate cyclase, Na+, K+ -ATPase, beta, muscarinic and ouabain receptors, also contained endogenous protein kinase activity. Phosphorylation of sarcolemmal membrane proteins by the endogenous protein kinase occurred mainly on 22 000 and 12 000 Mr proteins. To det...

journal_title：Journal of molecular and cellular cardiology

authors： Lee SW,Wallick ET,Schwartz A,Kranias EG

更新日期：1985-11-01 00:00:00

abstract：:To test a prediction that trans-sarcolemmal lactate movement is carrier mediated, 14C-labelled lactate or pyruvate and 3H-mannitol were transiently infused (2 min) into aerobically perfused rat hearts that had been depleted of intracellular monocarboxylates by a 30 min pre-perfusion in the absence of substrates. Uptak...

journal_title：Journal of molecular and cellular cardiology

authors： Dennis SC,Kohn MC,Anderson GJ,Garfinkel D

更新日期：1985-10-01 00:00:00

abstract：:A dose-response study concerning the importance of the flow rate (0.5 to 12 ml/min) and volume (2.5 to 60 ml) of calcium-free coronary perfusion (duration 5 min) in the induction of a calcium paradox on reperfusion (duration 15 min) with calcium-containing medium has been performed in the isolated rat heart (37 degree...

journal_title：Journal of molecular and cellular cardiology

authors： Oksendal AN,Jynge P,Sellevold OF,Rotevatn S,Saetersdal T

更新日期：1985-10-01 00:00:00

abstract：:Since systemic actions of thyroid hormone increase cardiac work, direct effects of T3 on myocardial protein turnover may be obscured in the intact animal. For this reason, the effects of T3 on synthesis and degradation of cellular protein were measured in replicate cultures of cardiac myocytes obtained from chick embr...

journal_title：Journal of molecular and cellular cardiology

authors： Carter WJ,van der Weijden Benjamin WS,Faas FH

更新日期：1985-09-01 00:00:00

abstract：:Monoclonal antibodies (MAb) to myosin heavy chains were prepared from one adult human ventricular myocardium. Several of these MAb reacted by indirect immunofluorescence in a heterogenous way on cryostat transverse sections of fibers from human atrial myocardium, suggesting the presence of different forms of myosin wi...

journal_title：Journal of molecular and cellular cardiology

authors： Dechesne C,Leger J,Bouvagnet P,Claviez M,Leger JJ

更新日期：1985-08-01 00:00:00

abstract：:Heart rate in mammals is inversely related to body weight. In the etruscan shrew it exceeds 960 beats/min. Since the heart depends primarily on energy from aerobic sources, it was interesting to examine some morphological parameters of the mitochondria in some mammals with a high heart frequency. Volume fraction and s...

journal_title：Journal of molecular and cellular cardiology

authors： Oron U,Mandelberg M

更新日期：1985-06-01 00:00:00

abstract：:We have compared and contrasted the actions of (-)isoproterenol and (+/-) trimetoquinol on rabbit heart preparations. In the presence of either GTP or Gpp[NH]p (guanosine-5'-(beta, gamma imino) triphosphate), trimetoquinol displayed partial agonist activity in stimulating adenylate cyclase activity in a particulate ra...

journal_title：Journal of molecular and cellular cardiology

authors： Babich M,Atkinson J,Piascik MT

更新日期：1985-06-01 00:00:00

abstract：:In the postnatal rat heart, muscle cells continue to divide as well as increase in size. At the same time the cells in the soleus muscle (a slow skeletal muscle) do not divide, although they continue to grow in size. Since microtubules may have a role in orienting intracellular structures in muscle, we determined the ...

journal_title：Journal of molecular and cellular cardiology

authors： Cartwright J Jr,Goldstein MA

更新日期：1985-01-01 00:00:00

abstract：:Coronary blood flow decreases cyclically in a partially occluded coronary artery of anesthetized dogs. Spontaneous aggregation and deaggregation of platelet plugs in the constricted artery have been indicated to be responsible for this phenomenon. A current hypothesis is that platelet aggregation may be determined by ...

journal_title：Journal of molecular and cellular cardiology

authors： Tada M,Esumi K,Yamagishi M,Kuzuya T,Matsuda H,Abe H,Uchida Y,Murao S

更新日期：1984-12-01 00:00:00

abstract：:The beta-receptors were isolated from rat cardiac myocytes and characterized. Isolated myocytes were prepared from adult rat hearts and characterized for viability. Membrane proteins were solubilized from myocytes with 1% Triton X-102. The solubilized membrane proteins were fractionated by DEAE-Sephacel ion exchange c...

journal_title：Journal of molecular and cellular cardiology

authors： Im JH,Puckett SW,Bowdon HR,Rogers WJ,Meezan E,Kim HD,Rackley CE

更新日期：1984-10-01 00:00:00

abstract：:We hypothesized that after a Ca2+-free period the magnitude of the Na+ gradient at the onset of Ca2+ reperfusion would grade the ensuing cell Ca2+ gain. Rabbit interventricular septa perfused with Hepes buffered solution (pH 7.4, [Ca2+] = 1.0 mM) and stimulated to contract isometrically at 60 min-1 at 30 degrees C wer...

journal_title：Journal of molecular and cellular cardiology

authors： Ruaño-Arroyo G,Gerstenblith G,Lakatta EG

更新日期：1984-09-01 00:00:00

abstract：:The resting potential of "sodium-loaded' cardiac cells can transiently hyperpolarize to levels negative to the steady state resting potential [3,5,6]. Hyperpolarization is associated with the coupled efflux of Na+ and influx of K+ driven by an active transport process and may result from an increased K+ equilibrium po...

journal_title：Journal of molecular and cellular cardiology

authors： Rasmussen HH,TenEick RE,McCullough JR,Singer DH

更新日期：1984-08-01 00:00:00

abstract：:Our previous work showed that myosin phosphorylation decreased the ATPase activity of skeletal muscle myofibrils that were lightly fixed with glutaraldehyde. The fixation process prevented sarcomere shortening and destruction of the ordered filament array upon the addition of ATP. We have now extended these results to...

journal_title：Journal of molecular and cellular cardiology

authors： Franks K,Cooke R,Stull JT

更新日期：1984-07-01 00:00:00

abstract：:Densitometric scanning of SDS-polyacrylamide gels was used to measure myosin heavy chain concentration in left ventricular specimens obtained from cat hearts 3 to 12 months after healing of small experimental myocardial infarctions. The study was designed to test the hypothesis that myosin concentration varies as a fu...

journal_title：Journal of molecular and cellular cardiology

authors： Kozlovskis PL,Silver JD,Rubin RW,Wong SS,Gaide MS,Bassett AL,Cameron JS,Myerburg RJ

更新日期：1984-06-01 00:00:00

abstract：:The effects of rate changes and 'premature' stimulation on the slow inward current of cardiac muscle were studied in the frog atrium. The effects of adrenaline under these conditions was investigated. It was found that adrenaline markedly accelerates the repriming kinetics of the slow inward channel. Adrenaline at low...

journal_title：Journal of molecular and cellular cardiology

authors： Shimoni Y,Raz S,Gotsman MS

更新日期：1984-05-01 00:00:00